Flow cytometric analysis of ENOPH1 expression in HAP-1 cells using anti-ENOPH1 antibody (Cat#61869, 1:1,000). Green, isotype control; red, ENOPH1.
Western blotting analysis using anti-ENOPH1 antibody (Cat#61869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ENOPH1 antibody (Cat#61869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ENOPH1 antibody (Cat#61869). ENOPH1 expression in wild type (WT) and ENOPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ENOPH1 antibody (Cat#61869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).