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Anti-Phospho-p38 (T180) Rabbit Monoclonal Antibody #4476

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Flow cytometric analysis of Phospho-p38 (T180) expression in C2C12 cells using anti-Phospho-p38 (T180) antibody (Cat#4476, 1:2,000). Green, isotype control; red, Phospho-p38 (T180).
Immunocytochemical staining of C2C12 cells with anti-phospho-p38 (T180) antibody (Cat#4476, 1:1,000). Nuclei were stained blue with anti-DAPI; Phospho-p38 (T180) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-p38 (T180) antibody (Cat#4476). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p38 (T180) antibody (Cat#4476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    MAPK14
  • 货号:
    4476
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Phospho-p38 (T180) Rabbit Monoclonal Antibody
Aliases MAPK14; Mitogen-Activated Protein Kinase 14; PRKM14; PRKM15; CSBP1; CSBP2; CSPB1; P38; Mitogen-Activated Protein Kinase P38 Alpha; MAX-Interacting Protein; CSAID-Binding Protein; MAP Kinase P38 Alpha; P38 MAP Kinase; MAP Kinase 14; EC 2.7.11.24; SAPK2A; Mxi2; CSBP; MXI2; Cytokine Suppressive Anti-Inflammatory Drug Binding Protein; Cytokine Suppressive Anti-Inflammatory Drug-Binding Protein; P38 Mitogen Activated Protein Kinase; Stress-Activated Protein Kinase 2A; Stress-Activated Protein Kinase 2a; MAP Kinase Mxi2; MAP Kinase MXI2; P38alpha Exip 3; EC 2.7.11; P38ALPHA; MAPK 14; SAPK2a; EXIP; RK
Background

Gene Name: MAPK14

NCBI Gene Entry: 1432

UniProt Entry: Q16539

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11410

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Phospho-p38 (T180)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-p38 (T180) expression in C2C12 cells using anti-Phospho-p38 (T180) antibody (Cat#4476, 1:2,000). Green, isotype control; red, Phospho-p38 (T180).
  • Immunocytochemical staining of C2C12 cells with anti-phospho-p38 (T180) antibody (Cat#4476, 1:1,000). Nuclei were stained blue with anti-DAPI; Phospho-p38 (T180) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-phospho-p38 (T180) antibody (Cat#4476). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p38 (T180) antibody (Cat#4476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Phospho-p38 (T180) expression in C2C12 cells using anti-Phospho-p38 (T180) antibody (Cat#4476, 1:2,000). Green, isotype control; red, Phospho-p38 (T180).
Immunocytochemical staining of C2C12 cells with anti-phospho-p38 (T180) antibody (Cat#4476, 1:1,000). Nuclei were stained blue with anti-DAPI; Phospho-p38 (T180) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-p38 (T180) antibody (Cat#4476). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p38 (T180) antibody (Cat#4476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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