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Anti-IDH1 Rabbit Monoclonal Antibody #4477

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Flow cytometric analysis of IDH1 expression in C2C12 cells using anti-IDH1 antibody (Cat#4477, 1:2,000). Green, isotype control; red, IDH1.

Immunocytochemical staining of HepG2 cells with anti-IDH1 antibody (Cat#4477, 1:1,000). Nuclei were stained blue with DAPI; IDH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-IDH1 antibody (Cat#4477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IDH1 antibody (Cat#4477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

IDH1
货号：

4477
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Picture

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Information

Product Name	Anti-IDH1 Rabbit Monoclonal Antibody
Aliases	IDH1; Isocitrate Dehydrogenase (NADP(+)) 1; Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic; Isocitrate Dehydrogenase 1 (NADP+), Soluble; Isocitrate Dehydrogenase [NADP] Cytoplasmic; Oxalosuccinate Decarboxylase; NADP(+)-Specific ICDH; EC 1.1.1.42; PICD; IDH; NADP-Dependent Isocitrate Dehydrogenase, Peroxisomal; NADP-Dependent Isocitrate Dehydrogenase, Cytosolic; Epididymis Secretory Sperm Binding Protein; Cytosolic NADP-Isocitrate Dehydrogenase; Isocitrate Dehydrogenase 1 (NADP+); Epididymis Secretory Protein Li 26; Epididymis Luminal Protein 216; HEL-S-26; HEL-216; IDCD; IDPC; IDPc; IDP
Background	Gene Name: IDH1 NCBI Gene Entry: 3417 UniProt Entry: O75874
Application Information	Molecular Weight: Predicted, 47 kDa; observed, 45 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11415 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human IDH1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of IDH1 expression in C2C12 cells using anti-IDH1 antibody (Cat#4477, 1:2,000). Green, isotype control; red, IDH1.
Immunocytochemical staining of HepG2 cells with anti-IDH1 antibody (Cat#4477, 1:1,000). Nuclei were stained blue with DAPI; IDH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-IDH1 antibody (Cat#4477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IDH1 antibody (Cat#4477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

IDH1
货号：

4477
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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