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Anti-Nudix hydrolase 1 Rabbit Monoclonal Antibody #4516

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Flow cytometric analysis of nudix hydrolase 1 expression in HeLa cells using anti-nudix hydrolase 1 antibody (Cat#4516, 1:2,000). Green, isotype control; red, nudix hydrolase 1.
Immunocytochemical staining of HeLa cells with anti-Nudix hydrolase 1 antibody (Cat#4516, 1:1,000). Nuclei were stained blue with DAPI; Nudix hydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-nudix hydrolase 1 antibody (Cat#4516). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nudix hydrolase 1 antibody (Cat#4516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    NUDT1
  • 货号:
    4516
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Nudix hydrolase 1 Rabbit Monoclonal Antibody
Aliases NUDT1; Nudix Hydrolase 1; 7,8-Dihydro-8-Oxoguanine Triphosphatase; Nudix Motif 1; 8-Oxo-DGTPase; MTH1; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 1; Nucleoside Diphosphate-Linked Moiety X-Type Motif 1; Methylated Purine Nucleoside Triphosphate Hydrolase; Oxidized Purine Nucleoside Triphosphate Hydrolase; 8-Oxo-7,8-Dihydrodeoxyguanosine Triphosphatase; Nucleoside Diphosphate-Linked Moiety X Motif 1; 8-Oxo-7,8-Dihydroguanosine Triphosphatase; 2-Hydroxy-DATP Diphosphatase; MutT Human Homolog 1; EC 3.6.1.56; EC 3.6.1.-
Background

Gene Name: NUDT1

NCBI Gene Entry: 4521

UniProt Entry: P36639

Application Information

Molecular Weight: Predicted, 18 kDa; observed, 18 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11610

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human MTH1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of nudix hydrolase 1 expression in HeLa cells using anti-nudix hydrolase 1 antibody (Cat#4516, 1:2,000). Green, isotype control; red, nudix hydrolase 1.
  • Immunocytochemical staining of HeLa cells with anti-Nudix hydrolase 1 antibody (Cat#4516, 1:1,000). Nuclei were stained blue with DAPI; Nudix hydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-nudix hydrolase 1 antibody (Cat#4516). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nudix hydrolase 1 antibody (Cat#4516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of nudix hydrolase 1 expression in HeLa cells using anti-nudix hydrolase 1 antibody (Cat#4516, 1:2,000). Green, isotype control; red, nudix hydrolase 1.
Immunocytochemical staining of HeLa cells with anti-Nudix hydrolase 1 antibody (Cat#4516, 1:1,000). Nuclei were stained blue with DAPI; Nudix hydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-nudix hydrolase 1 antibody (Cat#4516). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nudix hydrolase 1 antibody (Cat#4516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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