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Anti-Tribbles pseudokinase 3 Rabbit Monoclonal Antibody #4537

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Flow cytometric analysis of tribbles pseudokinase 3 expression in HT-1080 cells using anti-tribbles pseudokinase 3 antibody (Cat#4537, 1:2,000). Green, isotype control; red, tribbles pseudokinase 3.
Immunocytochemical staining of HT-1080 cells with anti-Tribbles pseudokinase 3 antibody (Cat#4537, 1:1,000). Nuclei were stained blue with DAPI; Tribbles pseudokinase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
Western blotting analysis using anti-tribbles pseudokinase 3 antibody (Cat#4537). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-tribbles pseudokinase 3 antibody (Cat#4537, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    TRIB3
  • 货号:
    4537
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Tribbles pseudokinase 3 Rabbit Monoclonal Antibody
Aliases TRIB3; Tribbles Pseudokinase 3; TRB3; P65-Interacting Inhibitor Of NF-Kappa-B; Tribbles Homolog 3; DJ1103G7.3; C20orf97; SKIP3; TRB-3; NIPK; SINK; Neuronal Cell Death Inducible Putative Kinase; Neuronal Cell Death-Inducible Putative Kinase; P65-Interacting Inhibitor Of NF-KappaB; Chromosome 20 Open Reading Frame 97; Tribbles Homolog 3 (Drosophila); C20ORF97
Background

Gene Name: TRIB3

NCBI Gene Entry: 57761

UniProt Entry: Q96RU7

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11715

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human TRIB3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of tribbles pseudokinase 3 expression in HT-1080 cells using anti-tribbles pseudokinase 3 antibody (Cat#4537, 1:2,000). Green, isotype control; red, tribbles pseudokinase 3.
  • Immunocytochemical staining of HT-1080 cells with anti-Tribbles pseudokinase 3 antibody (Cat#4537, 1:1,000). Nuclei were stained blue with DAPI; Tribbles pseudokinase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
  • Western blotting analysis using anti-tribbles pseudokinase 3 antibody (Cat#4537). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-tribbles pseudokinase 3 antibody (Cat#4537, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of tribbles pseudokinase 3 expression in HT-1080 cells using anti-tribbles pseudokinase 3 antibody (Cat#4537, 1:2,000). Green, isotype control; red, tribbles pseudokinase 3.
Immunocytochemical staining of HT-1080 cells with anti-Tribbles pseudokinase 3 antibody (Cat#4537, 1:1,000). Nuclei were stained blue with DAPI; Tribbles pseudokinase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
Western blotting analysis using anti-tribbles pseudokinase 3 antibody (Cat#4537). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-tribbles pseudokinase 3 antibody (Cat#4537, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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