Flow cytometric analysis of P70 S6 Kinase beta expression in HeLa cells using P70 S6 Kinase beta antibody (Cat#62521, 1:2,000). Green, isotype control; red, P70 S6 Kinase beta.
Immunocytochemical staining of HeLa cells with anti-P70 S6 Kinase beta antibody (Cat#62521, 1:1,000). Nuclei were stained blue with DAPI; P70 S6 Kinase beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-P70 S6 Kinase beta antibody (Cat#62521). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-R70 S6 Kinase beta antibody (Cat#62521, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-P70 S6 Kinase beta antibody (Cat#62521). P70 S6 Kinase beta expression in wild type (WT) and P70 S6 Kinase beta shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-P70 S6 Kinase beta antibody (Cat#62521, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of P70 S6 Kinase beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-P70 S6 Kinase beta antibody (Cat#62521, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-P70 S6 Kinase beta antibody (Cat#62521, 1:1,000), Top panel: wild-type (WT); Bottom panal: P70 S6 Kinase beta shRNA knockdown (KD). Nuclei were stained blue with DAPI; P70 S6 Kinase beta was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
