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Anti-HIF1AN Rabbit Monoclonal Antibody #4546

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Flow cytometric analysis of HIF1AN expression in HeLa cells using anti-HIF1AN antibody (Cat#4546, 1:2,000). Green, isotype control; red, HIF1AN.

Immunocytochemical staining of HeLa cells with anti-HIF1AN antibody (Cat#4546, 1:1,000). Nuclei were stained blue with DAPI; HIF1AN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-HIF1AN antibody (Cat#4546). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF1AN antibody (Cat#4546, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

HIF1AN
货号：

4546
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-HIF1AN Rabbit Monoclonal Antibody
Aliases	HIF1AN; Hypoxia Inducible Factor 1 Subunit Alpha Inhibitor; FIH1; Hypoxia-Inducible Factor Asparagine Hydroxylase; Hypoxia-Inducible Factor 1-Alpha Inhibitor; Peptide-Aspartate Beta-Dioxygenase; Factor Inhibiting HIF-1; FLJ20615; FLJ22027; FIH-1; Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor; Factor Inhibiting HIF1; DKFZp762F1811; DKFZP762F1811; EC 1.14.11.30; EC 1.14.11.N4
Background	Gene Name: HIF1AN NCBI Gene Entry: 55662 UniProt Entry: Q9NWT6
Application Information	Molecular Weight: Predicted, 40 kDa; observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11760 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HIF1AN
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of HIF1AN expression in HeLa cells using anti-HIF1AN antibody (Cat#4546, 1:2,000). Green, isotype control; red, HIF1AN.
Immunocytochemical staining of HeLa cells with anti-HIF1AN antibody (Cat#4546, 1:1,000). Nuclei were stained blue with DAPI; HIF1AN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HIF1AN antibody (Cat#4546). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF1AN antibody (Cat#4546, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

HIF1AN
货号：

4546
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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