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Anti-NEK2 Rabbit Monoclonal Antibody #4547

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Flow cytometric analysis of NEK2 expression in HepG2 cells using anti-NEK2 antibody (Cat#4547, 1:2,000). Green, isotype control; red, NEK2.
Immunocytochemical staining of HepG2 cells with anti-NEK2 antibody (Cat#4547, 1:1,000). Nuclei were stained blue with DAPI; NEK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-NEK2 antibody (Cat#4547). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK2 antibody (Cat#4547, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    NEK2
  • 货号：
    4547
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-NEK2 Rabbit Monoclonal Antibody
Aliases NEK2; NIMA Related Kinase 2; NEK2A; NLK1; PPP1R111; RP67; NIMA (Never In Mitosis Gene A)-Related Kinase 2; Protein Phosphatase 1, Regulatory Subunit 111; Serine/Threonine-Protein Kinase Nek2; NimA-Related Protein Kinase; NimA-Like Protein Kinase 1; EC 2.7.11.1; Never In Mitosis A-Related Kinase; EC 2.7.11; HsPK 21; HSPK 21; HsPK21
Background

Gene Name: NEK2

NCBI Gene Entry: 4751

UniProt Entry: P51955
Application Information

Molecular Weight: Predicted, 52 kDa; observed, 48 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11765

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human NEK2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NEK2 expression in HepG2 cells using anti-NEK2 antibody (Cat#4547, 1:2,000). Green, isotype control; red, NEK2.
  • Immunocytochemical staining of HepG2 cells with anti-NEK2 antibody (Cat#4547, 1:1,000). Nuclei were stained blue with DAPI; NEK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-NEK2 antibody (Cat#4547). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK2 antibody (Cat#4547, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    NEK2
  • 货号：
    4547
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of NEK2 expression in HepG2 cells using anti-NEK2 antibody (Cat#4547, 1:2,000). Green, isotype control; red, NEK2.
Immunocytochemical staining of HepG2 cells with anti-NEK2 antibody (Cat#4547, 1:1,000). Nuclei were stained blue with DAPI; NEK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-NEK2 antibody (Cat#4547). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK2 antibody (Cat#4547, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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