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Anti-p150 CAF1 Rabbit Monoclonal Antibody #4566

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Flow cytometric analysis of p150CAF1 expression in HepG2 cells using anti-p150CAF1 antibody (Cat#4566, 1:2,000). Green, isotype control; red, p150CAF1.
Immunocytochemical staining of HepG2 cells with anti-P150 CAF1 antibody (Cat#4566, 1:1,000) . Nuclei were stained blue with DAPI; P150 CAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-p150 CAF1 antibody (Cat#4566). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p150 CAF1 antibody (Cat#4566, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    CHAF1A
  • 货号:
    4566
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-p150 CAF1 Rabbit Monoclonal Antibody
Aliases CHAF1A; Chromatin Assembly Factor 1 Subunit A; CAF1P150; CAF1B; CAF-1; CAF1; P150; Chromatin Assembly Factor I P150 Subunit; Chromatin Assembly Factor I (150 KDa); CAF-I 150 KDa Subunit; CAF-1 Subunit A; CAF-I P150; MGC71229; Hp150; Chromatin Assembly Factor 1, Subunit A (P150); CTB-50L17.7; CAF
Background

Gene Name: CHAF1A

NCBI Gene Entry: 10036

UniProt Entry: Q13111

Application Information

Molecular Weight: Predicted, 107 kDa; observed, 150 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11855

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry(FCM)

Immunogen A synthesized peptide derived from human CAF 1 subunit A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:200 - 1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of p150CAF1 expression in HepG2 cells using anti-p150CAF1 antibody (Cat#4566, 1:2,000). Green, isotype control; red, p150CAF1.
  • Immunocytochemical staining of HepG2 cells with anti-P150 CAF1 antibody (Cat#4566, 1:1,000) . Nuclei were stained blue with DAPI; P150 CAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-p150 CAF1 antibody (Cat#4566). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p150 CAF1 antibody (Cat#4566, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of p150CAF1 expression in HepG2 cells using anti-p150CAF1 antibody (Cat#4566, 1:2,000). Green, isotype control; red, p150CAF1.
Immunocytochemical staining of HepG2 cells with anti-P150 CAF1 antibody (Cat#4566, 1:1,000) . Nuclei were stained blue with DAPI; P150 CAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-p150 CAF1 antibody (Cat#4566). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p150 CAF1 antibody (Cat#4566, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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