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Anti-MCM3 Rabbit Monoclonal Antibody #4583

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Flow cytometric analysis of MCM3 expression in HepG2 cells using anti-MCM3 antibody (Cat#4583, 1:2,000). Green, isotype control; red, MCM3.

Immunocytochemical staining of HepG2 cells with anti-MCM3 antibody (Cat#4583, 1:1,000). Nuclei were stained blue with DAPI;
MCM3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-MCM3 antibody (Cat#4583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM3 antibody (Cat#4583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

MCM3
货号：

4583
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-MCM3 Rabbit Monoclonal Antibody
Aliases	MCM3; Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; DNA Replication Licensing Factor MCM3; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3; Cervical Cancer Proto-Oncogene 5; DNA Replication Factor MCM3; HRlf Beta Subunit; EC 3.6.4.12; HCC5; P1.H; RLFB
Background	Gene Name: MCM3 NCBI Gene Entry: 4172 UniProt Entry: P25205
Application Information	Molecular Weight: Predicted, 91 kDa; observed, 100 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11930 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human MCM3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of MCM3 expression in HepG2 cells using anti-MCM3 antibody (Cat#4583, 1:2,000). Green, isotype control; red, MCM3.
Immunocytochemical staining of HepG2 cells with anti-MCM3 antibody (Cat#4583, 1:1,000). Nuclei were stained blue with DAPI;
MCM3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MCM3 antibody (Cat#4583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM3 antibody (Cat#4583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

MCM3
货号：

4583
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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