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Anti-Actin like 6A Rabbit Monoclonal Antibody #4593

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Flow cytometric analysis of actin like 6A expression in HT-1080 cells using anti-actin like 6A antibody (Cat#4593, 1:2,000). Green, isotype control; red, actin like 6A.
Immunocytochemical staining of HT-1080 cells with anti-Actin like 6A antibody (Cat#4593, 1:1,000). Nuclei were stained blue with DAPI; Actin like 6A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-actin like 6A antibody (Cat#4593). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-actin like 6A antibody (Cat#4593, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    ACTL6A
  • 货号:
    4593
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Actin like 6A Rabbit Monoclonal Antibody
Aliases ACTL6A; Actin Like 6A; BAF53A; INO80K; BRG1-Associated Factor 53A; INO80 Complex Subunit K; SMARCN1; 53 KDa BRG1-Associated Factor A; Actin-Related Protein Baf53a; BAF Complex 53 KDa Subunit; Actin-Related Protein; Actin-Like Protein 6A; ArpNbeta; ACTL6; BAF53; Arp4; HArpN Beta; ARPN-BETA; Baf53a; Actl6; ARP4
Background

Gene Name: ACTL6A

NCBI Gene Entry: 86

UniProt Entry: O96019

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11980

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human ACTL6A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of actin like 6A expression in HT-1080 cells using anti-actin like 6A antibody (Cat#4593, 1:2,000). Green, isotype control; red, actin like 6A.
  • Immunocytochemical staining of HT-1080 cells with anti-Actin like 6A antibody (Cat#4593, 1:1,000). Nuclei were stained blue with DAPI; Actin like 6A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-actin like 6A antibody (Cat#4593). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-actin like 6A antibody (Cat#4593, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of actin like 6A expression in HT-1080 cells using anti-actin like 6A antibody (Cat#4593, 1:2,000). Green, isotype control; red, actin like 6A.
Immunocytochemical staining of HT-1080 cells with anti-Actin like 6A antibody (Cat#4593, 1:1,000). Nuclei were stained blue with DAPI; Actin like 6A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-actin like 6A antibody (Cat#4593). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-actin like 6A antibody (Cat#4593, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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