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Anti-MCM7 Rabbit Monoclonal Antibody #4633

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Flow cytometric analysis of MCM7 expression in HepG2 cells using anti-MCM7 antibody (Cat#4633, 1:2,000). Green, isotype control; red, MCM7.
Immunocytochemical staining of HepG2 cells with anti-MCM7 antibody (Cat#4633, 1:1,000) . Nuclei were stained blue with DAPI; MCM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MCM7 antibody (Cat#4633). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM7 antibody (Cat#4633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    MCM7
  • 货号：
    4633
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-MCM7 Rabbit Monoclonal Antibody
Aliases Minichromosome Maintenance Complex Component 7; CDC47; PPP1R104; MCM2; Protein Phosphatase 1, Regulatory Subunit 104; DNA Replication Licensing Factor MCM7; CDC47 Homolog; P1.1-MCM3; MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 7; Minichromosome Maintenance Deficient 7; Homolog Of S. Cerevisiae Cdc47; EC 3.6.4.12; P1CDC47; PNAS146; P85MCM
Background

Gene Name: MCM7

NCBI Gene Entry: 4176

UniProt Entry: P33993
Application Information

Molecular Weight: Predicted, 81 kDa; observed, 81 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12175

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human MCM7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MCM7 expression in HepG2 cells using anti-MCM7 antibody (Cat#4633, 1:2,000). Green, isotype control; red, MCM7.
  • Immunocytochemical staining of HepG2 cells with anti-MCM7 antibody (Cat#4633, 1:1,000) . Nuclei were stained blue with DAPI; MCM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-MCM7 antibody (Cat#4633). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM7 antibody (Cat#4633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    MCM7
  • 货号：
    4633
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of MCM7 expression in HepG2 cells using anti-MCM7 antibody (Cat#4633, 1:2,000). Green, isotype control; red, MCM7.
Immunocytochemical staining of HepG2 cells with anti-MCM7 antibody (Cat#4633, 1:1,000) . Nuclei were stained blue with DAPI; MCM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MCM7 antibody (Cat#4633). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM7 antibody (Cat#4633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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