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Anti-ISG15 Rabbit Monoclonal Antibody #4638

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Immunocytochemical staining of HepG2 cells with anti-ISG15 antibody (Cat#4638, 1:1,000) . Nuclei were stained blue with DAPI; ISG15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-ISG15 antibody (Cat#4638). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISG15 antibody (Cat#4638, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    ISG15
  • 货号：
    4638
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-ISG15 Rabbit Monoclonal Antibody
Aliases ISG15; ISG15 Ubiquitin Like Modifier; UCRP; IFI15; G1P2; Interferon, Alpha-Inducible Protein (Clone IFI-15K); Ubiquitin Cross-Reactive Protein; Ubiquitin-Like Protein ISG15; HUCRP; IP17; Interferon-Induced 17-KDa/15-KDa Protein; Interferon-Stimulated Protein, 15 KDa; Interferon-Induced 15 KDa Protein; Interferon-Induced 17 KDa Protein; IMD38
Background

Gene Name: ISG15

NCBI Gene Entry: 9636

UniProt Entry: P05161
Application Information

Molecular Weight: Predicted, 18 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12200

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ISG15
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-ISG15 antibody (Cat#4638, 1:1,000) . Nuclei were stained blue with DAPI; ISG15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-ISG15 antibody (Cat#4638). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISG15 antibody (Cat#4638, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    ISG15
  • 货号：
    4638
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunocytochemical staining of HepG2 cells with anti-ISG15 antibody (Cat#4638, 1:1,000) . Nuclei were stained blue with DAPI; ISG15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-ISG15 antibody (Cat#4638). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISG15 antibody (Cat#4638, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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