Flow cytometric analysis of NEK7 expression in HepG2 cells using anti-NEK7 antibody (Cat#4658, 1:2,000). Green, isotype control; red, NEK7.
Immunocytochemical staining of HepG2 cells with anti-NEK7 antibody (Cat#4658, 1:1,000). Nuclei were stained blue with DAPI; NEK7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NEK7 antibody (Cat#4658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK7 antibody (Cat#4658, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
NIMA Related Kinase 7; NIMA (Never In Mitosis Gene A)-Related Kinase 7; Serine/Threonine-Protein Kinase Nek7; Never In Mitosis A-Related Kinase 7; NimA-Related Protein Kinase 7; EC 2.7.11.34; EC 2.7.11.1
Flow cytometric analysis of NEK7 expression in HepG2 cells using anti-NEK7 antibody (Cat#4658, 1:2,000). Green, isotype control; red, NEK7.
Immunocytochemical staining of HepG2 cells with anti-NEK7 antibody (Cat#4658, 1:1,000). Nuclei were stained blue with DAPI; NEK7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NEK7 antibody (Cat#4658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK7 antibody (Cat#4658, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of NEK7 expression in HepG2 cells using anti-NEK7 antibody (Cat#4658, 1:2,000). Green, isotype control; red, NEK7.
Immunocytochemical staining of HepG2 cells with anti-NEK7 antibody (Cat#4658, 1:1,000). Nuclei were stained blue with DAPI; NEK7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NEK7 antibody (Cat#4658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK7 antibody (Cat#4658, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
