Flow cytometric analysis of NEK6 expression in HT-1080 cells using anti-NEK6 antibody (Cat#4659, 1:2,000). Green, isotype control; red, NEK6.
Immunocytochemical staining of HT-1080 cells with anti-NEK6 antibody (Cat#4659, 1:1,000). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NEK6 antibody (Cat#4659). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK6 antibody (Cat#4659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Anti-NIMA Related Kinase 6 Rabbit Monoclonal Antibody
Aliases
NIMA Related Kinase 6; SID6-1512; NIMA (Never In Mitosis Gene A)-Related Kinase 6; Putative Serine-Threonine Protein Kinase; Serine/Threonine-Protein Kinase Nek6; Never In Mitosis A-Related Kinase 6; NimA-Related Protein Kinase 6; Protein Kinase SID6-1512; EC 2.7.11.34; EC 2.7.11.1
Flow cytometric analysis of NEK6 expression in HT-1080 cells using anti-NEK6 antibody (Cat#4659, 1:2,000). Green, isotype control; red, NEK6.
Immunocytochemical staining of HT-1080 cells with anti-NEK6 antibody (Cat#4659, 1:1,000). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NEK6 antibody (Cat#4659). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK6 antibody (Cat#4659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of NEK6 expression in HT-1080 cells using anti-NEK6 antibody (Cat#4659, 1:2,000). Green, isotype control; red, NEK6.
Immunocytochemical staining of HT-1080 cells with anti-NEK6 antibody (Cat#4659, 1:1,000). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NEK6 antibody (Cat#4659). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK6 antibody (Cat#4659, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
