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KD-Validated Anti-ABCF2 Mouse Monoclonal Antibody #63583

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Flow cytometric analysis of ABCF2 expression in C2C12 cells using anti-ABCF2 antibody (Cat#63583, 1:2,000). Green, isotype control; red, ABCF2.
Western blotting analysis using anti-ABCF2 antibody (Cat#63583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ABCF2 antibody (Cat#63583, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ABCF2 antibody (Cat#63583). ABCF2 expression in wild type (WT) and ABCF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ABCF2 antibody (Cat#63583, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    ABCF2
  • 货号:
    63583
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-ABCF2 Mouse Monoclonal Antibody
Aliases ABCF2; ATP Binding Cassette Subfamily F Member 2; EST133090; HUSSY-18; ABC28; ATP-Binding Cassette, Sub-Family F (GCN20), Member 2; ATP-Binding Cassette Sub-Family F Member 2; Iron-Inhibited ABC Transporter 2; M-ABC1; ABC-Type Transport Protein; EC 3.6.3.17; EC 3.6.3; HUSSY18
Background

Gene Name: ABCF2

NCBI Gene Entry: 10061

UniProt Entry: Q9UG63

Application Information

Molecular Weight: Predicted, 71 kDa; observed, 71 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6635

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human ABCF2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ABCF2 expression in C2C12 cells using anti-ABCF2 antibody (Cat#63583, 1:2,000). Green, isotype control; red, ABCF2.
  • Western blotting analysis using anti-ABCF2 antibody (Cat#63583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ABCF2 antibody (Cat#63583, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-ABCF2 antibody (Cat#63583). ABCF2 expression in wild type (WT) and ABCF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ABCF2 antibody (Cat#63583, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of ABCF2 expression in C2C12 cells using anti-ABCF2 antibody (Cat#63583, 1:2,000). Green, isotype control; red, ABCF2.
Western blotting analysis using anti-ABCF2 antibody (Cat#63583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ABCF2 antibody (Cat#63583, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ABCF2 antibody (Cat#63583). ABCF2 expression in wild type (WT) and ABCF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ABCF2 antibody (Cat#63583, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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