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Anti-Dihydrolipoamide dehydrogenase Rabbit Monoclonal Antibody #4662

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Flow cytometric analysis of dihydrolipoamide dehydrogenase expression in HT-1080 cells using anti-dihydrolipoamide dehydrogenase antibody (Cat#4662, 1:2,000). Green, isotype control; red, dihydrolipoamide dehydrogenase.

Immunocytochemical staining of HT-1080 cells with anti-dihydrolipoamide dehydrogenase antibody (Cat#4662, 1:1,000). Nuclei were stained blue with DAPI; Dihydrolipoamide dehydrogenase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-dihydrolipoamide dehydrogenase antibody (Cat#4662). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dihydrolipoamide dehydrogenase antibody (Cat#4662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

DLD
货号：

4662
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-Dihydrolipoamide dehydrogenase Rabbit Monoclonal Antibody
Aliases	DLD; Dihydrolipoamide Dehydrogenase; OGDC-E3; DLDH; GCSL; LAD; E3; E3 Component Of Pyruvate Dehydrogenase Complex, 2-Oxo-Glutarate Complex, Branched Chain Keto Acid Dehydrogenase Complex; Dihydrolipoyl Dehydrogenase, Mitochondrial; Glycine Cleavage System L Protein; EC 1.8.1.4; PHE3; Dihydrolipoamide Dehydrogenase (E3 Component Of Pyruvate Dehydrogenase Complex, 2-Oxo-Glutarate Complex, Branched Chain Keto Acid Dehydrogenase Complex); Epididymis Secretory Sperm Binding Protein; Glycine Cleavage System Protein L; Lipoamide Dehydrogenase; Lipoyl Dehydrogenase; Lipoamide Reductase; Diaphorase; EC 1.8.1 48; DLDD
Background	Gene Name: DLD NCBI Gene Entry: 1738 UniProt Entry: P09622
Application Information	Molecular Weight: Predicted, 54 kDa; observed, 56 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12320 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human DLDH
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of dihydrolipoamide dehydrogenase expression in HT-1080 cells using anti-dihydrolipoamide dehydrogenase antibody (Cat#4662, 1:2,000). Green, isotype control; red, dihydrolipoamide dehydrogenase.
Immunocytochemical staining of HT-1080 cells with anti-dihydrolipoamide dehydrogenase antibody (Cat#4662, 1:1,000). Nuclei were stained blue with DAPI; Dihydrolipoamide dehydrogenase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-dihydrolipoamide dehydrogenase antibody (Cat#4662). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dihydrolipoamide dehydrogenase antibody (Cat#4662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

DLD
货号：

4662
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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