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Anti-Caspase 9 Rabbit Monoclonal Antibody #4678

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Flow cytometric analysis of Caspase 9 expression in HepG2 cells using anti-Caspase 9 antibody (Cat#4678, 1:2,000). Green, isotype control; red, Caspase 9.

Immunocytochemical staining of HepG2 cells with anti-caspase 9 antibody (Cat#4678, 1:1,000) . Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-caspase 9 antibody (Cat#4678). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 9 antibody (Cat#4678, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CASP9
货号：

4678
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Caspase 9 Rabbit Monoclonal Antibody
Aliases	CASP9; Caspase 9; Apoptosis-Related Cysteine Peptidase; Regulatory Subunit 56; Apoptotic Protease Mch-6; PPP1R56; APAF-3; Apoptotic Protease Activating Factor 3; ICE-LAP6; caspase-9; MCH6; Protein Phosphatase 1; Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; CASP-9; APAF3; EC 3.4.22.62; Caspase 9; Apoptosis-Related Cysteine Protease; Apoptotic Protease-Activating Factor 3; Protein Phosphatase 1
Background	Gene Name: CASP9 NCBI Gene Entry: 842 UniProt Entry: P55211
Application Information	Molecular Weight: Predicted, 46 kDa; observed, 46 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12400 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human Caspase-9
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Caspase 9 expression in HepG2 cells using anti-Caspase 9 antibody (Cat#4678, 1:2,000). Green, isotype control; red, Caspase 9.
Immunocytochemical staining of HepG2 cells with anti-caspase 9 antibody (Cat#4678, 1:1,000) . Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-caspase 9 antibody (Cat#4678). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 9 antibody (Cat#4678, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CASP9
货号：

4678
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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