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Anti-G3BP1 Rabbit Monoclonal Antibody #4716

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Flow cytometric analysis of G3BP1 expression in HepG2 cells using anti-G3BP1 antibody (Cat#4716, 1:2,000). Green, isotype control; red, G3BP1.

Immunocytochemical staining of HepG2 cells with anti-G3BP1 antibody (Cat#4716, 1:1,000) . Nuclei were stained blue with DAPI; G3BP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-G3BP1 antibody (Cat#4716). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G3BP1 antibody (Cat#4716, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

G3BP1
货号：

4716
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-G3BP1 Rabbit Monoclonal Antibody
Aliases	G3BP1; G3BP Stress Granule Assembly Factor 1; G3BP; HDH-VIII; Ras-GTPase-Activating Protein SH3-Domain-Binding Protein; GTPase Activating Protein (SH3 Domain) Binding Protein 1; Ras GTPase-Activating Protein-Binding Protein 1; GAP SH3 Domain-Binding Protein 1; ATP-Dependent DNA Helicase VIII; DNA Helicase VIII; G3BP-1; RasGAP-Associated Endoribonuclease G3BP; GAP Binding Protein; EC 3.6.4.12; EC 3.6.4.13; HDH VIII; EC 3.6.1
Background	Gene Name: G3BP1 NCBI Gene Entry: 10146 UniProt Entry: Q13283
Application Information	Molecular Weight: Predicted, 52 kDa; observed, 68 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12590 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human G3BP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of G3BP1 expression in HepG2 cells using anti-G3BP1 antibody (Cat#4716, 1:2,000). Green, isotype control; red, G3BP1.
Immunocytochemical staining of HepG2 cells with anti-G3BP1 antibody (Cat#4716, 1:1,000) . Nuclei were stained blue with DAPI; G3BP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-G3BP1 antibody (Cat#4716). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G3BP1 antibody (Cat#4716, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

G3BP1
货号：

4716
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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