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Anti-PDPK1 Rabbit Monoclonal Antibody #4732

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Flow cytometric analysis of PDPK1 expression in HepG2 cells using anti-PDPK1 antibody (Cat#4732, 1:2,000). Green, isotype control; red, PDPK1.

Immunocytochemical staining of HepG2 cells with anti-PDPK1 antibody (Cat#4732, 1:1,000) . Nuclei were stained blue with DAPI; PDPK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PDPK1 antibody (Cat#4732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDPK1 antibody (Cat#4732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PDPK1
货号：

4732
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-PDPK1 Rabbit Monoclonal Antibody
Aliases	PDPK1; 3-Phosphoinositide Dependent Protein Kinase 1; PDK1; 3-Phosphoinositide-Dependent Protein Kinase 1; EC 2.7.11.1; 3-Phosphoinositide-Dependent Protein Kinase 2 Pseudogene; Putative 3-Phosphoinositide-Dependent Protein Kinase; PkB Kinase Like Gene 1; PkB Kinase; EC 2.7.11; PRO0461; PDPK2P; PDPK2; HPDK1
Background	Gene Name: PDPK1 NCBI Gene Entry: 5170 UniProt Entry: O15530
Application Information	Molecular Weight: Predicted, 63 kDa; observed, 52-65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12670 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PDPK1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PDPK1 expression in HepG2 cells using anti-PDPK1 antibody (Cat#4732, 1:2,000). Green, isotype control; red, PDPK1.
Immunocytochemical staining of HepG2 cells with anti-PDPK1 antibody (Cat#4732, 1:1,000) . Nuclei were stained blue with DAPI; PDPK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PDPK1 antibody (Cat#4732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDPK1 antibody (Cat#4732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PDPK1
货号：

4732
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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