Anti-Phospho-HSF1 (Ser303/Ser307) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-HSF1 (Ser303/Ser307) Rabbit Monoclonal Antibody #4748

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Flow cytometric analysis of phospho-HSF1 (Ser303/Ser307) expression in HepG2 cells using anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#4748, 1:2,000). Green, isotype control; red, phospho-HSF1 (Ser303/Ser307).

Immunocytochemical staining of HepG2 cells with anti-Phospho-HSF1 (Ser303/Ser307) antibody (Cat#4748, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-HSF1 (Ser303/Ser307) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#4748). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#4748, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

HSF1
货号：

4748
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Phospho-HSF1 (Ser303/Ser307) Rabbit Monoclonal Antibody
Aliases	HSF1; Heat Shock Transcription Factor 1; HSTF1; Heat Shock Factor Protein 1; HSTF; HSF
Background	Gene Name: HSF1 NCBI Gene Entry: 3297 UniProt Entry: Q00613
Application Information	Molecular Weight: Predicted, 57 kDa; observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12750 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-HSF1 (Ser303/Ser307)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of phospho-HSF1 (Ser303/Ser307) expression in HepG2 cells using anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#4748, 1:2,000). Green, isotype control; red, phospho-HSF1 (Ser303/Ser307).
Immunocytochemical staining of HepG2 cells with anti-Phospho-HSF1 (Ser303/Ser307) antibody (Cat#4748, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-HSF1 (Ser303/Ser307) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#4748). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-HSF1 (Ser303/Ser307) antibody (Cat#4748, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

HSF1
货号：

4748
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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