Flow cytometric analysis of epidermal growth factor receptor expression in HepG2 cells using anti-epidermal growth factor receptor antibody (Cat#4795, 1:2,000). Green, isotype control; red, epidermal growth factor receptor.
Immunocytochemical staining of HepG2 cells with anti-Epidermal growth factor receptor antibody (Cat#4795, 1:1,000). Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-epidermal growth factor receptor antibody (Cat#4795). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epidermal growth factor receptor antibody (Cat#4795, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).