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Anti-ACE Rabbit Monoclonal Antibody #4887

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Immunocytochemical staining of C2C12 cells with anti-ACE antibody (Cat#4887, 1:1,000) . Nuclei were stained blue with DAPI; ACE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-ACE antibody (Cat#4887). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACE antibody (Cat#4887, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    ACE
  • 货号：
    4887
  • 应用：
    WB, IC
  • 物种反应性：
    M
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-ACE Rabbit Monoclonal Antibody
Aliases ACE; Angiotensin I Converting Enzyme; CD143; ACE1; DCP1; Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1; Angiotensin-Converting Enzyme; Dipeptidyl Carboxypeptidase I; CD143 Antigen; Kininase II; DCP; Dipeptidyl Carboxypeptidase 1; Peptidyl-Dipeptidase A; Carboxycathepsin; Peptidase P; EC 3.4.15.
Background

Gene Name: ACE

NCBI Gene Entry: 1636

UniProt Entry: P12821
Application Information

Molecular Weight: Predicted, 150 kDa; observed, 180 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13595

Species Reactivity: Mouse

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen Recombinant protein of mouse Angiotensin Converting Enzyme 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of C2C12 cells with anti-ACE antibody (Cat#4887, 1:1,000) . Nuclei were stained blue with DAPI; ACE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-ACE antibody (Cat#4887). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACE antibody (Cat#4887, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    ACE
  • 货号：
    4887
  • 应用：
    WB, IC
  • 物种反应性：
    M
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunocytochemical staining of C2C12 cells with anti-ACE antibody (Cat#4887, 1:1,000) . Nuclei were stained blue with DAPI; ACE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-ACE antibody (Cat#4887). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACE antibody (Cat#4887, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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