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KD-Validated Anti-WWOX Rabbit Monoclonal Antibody #65666

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Western blotting analysis using anti-WWOX antibody (Cat#65666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WWOX antibody (Cat#65666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-WWOX antibody (Cat#65666). WWOX expression in wild-type (WT) and WWOX shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-WWOX antibody (Cat#65666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of WWOX expression in HeLa cells using anti-WWOX antibody (Cat#65666, 1:2,000). Green, isotype control; red, WWOX.
  • 基因名:
    WWOX
  • 货号:
    65666
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-WWOX Rabbit Monoclonal Antibody
Aliases WWOX; WW Domain Containing Oxidoreductase; SDR41C1; WOX1; FOR; WW Domain-Containing Oxidoreductase; Short Chain Dehydrogenase/Reductase Family 41C Member 1; Fragile Site FRA16D Oxidoreductase; Short Chain Dehydrogenase/Reductase Family 41C, Member 1; WW Domain-Containing Protein WWOX; EC 1.1.1.-; D16S432E; EC 1.1.1; HHCMA56; PRO0128; EIEE28; FRA16D; SCAR12; DEE28
Background

Gene Name: WWOX

NCBI Gene Entry: 51741

UniProt Entry: Q9NZC7

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4780

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human WWOX
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-WWOX antibody (Cat#65666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WWOX antibody (Cat#65666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-WWOX antibody (Cat#65666). WWOX expression in wild-type (WT) and WWOX shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-WWOX antibody (Cat#65666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of WWOX expression in HeLa cells using anti-WWOX antibody (Cat#65666, 1:2,000). Green, isotype control; red, WWOX.
Citation(0)
Western blotting analysis using anti-WWOX antibody (Cat#65666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WWOX antibody (Cat#65666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-WWOX antibody (Cat#65666). WWOX expression in wild-type (WT) and WWOX shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-WWOX antibody (Cat#65666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of WWOX expression in HeLa cells using anti-WWOX antibody (Cat#65666, 1:2,000). Green, isotype control; red, WWOX.
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