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Anti-ECA39 Rabbit Polyclonal Antibody #50181

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Immunocytochemical analysis of ECA39 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of ECA39 expression in Jurkat (A), Ramos (B), NIH3T3 (C), PC12 (D) whole cell lysates.
  • 基因名：
    BCAT1
  • 货号：
    50181
  • 应用：
    WB, IC
  • 物种反应性：
    H, Mk, R, B, D, P, Rb, S
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-ECA39 Rabbit Polyclonal Antibody
Aliases BCT1; ECA39; Branched-chain-amino-acid aminotransferase, cytosolic; BCAT(c); Protein ECA39
Background

Gene Name: BCAT1

NCBI Gene Entry: 586

UniProt Entry: P54687
Application Information

Molecular Weight: Predicted, 42 kDa; observed, 42 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, monkey, rat, bovine, dog, pig, monkey, rabbit, sheep

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ECA39
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:50-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of ECA39 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of ECA39 expression in Jurkat (A), Ramos (B), NIH3T3 (C), PC12 (D) whole cell lysates.
Citation(0)
  • 基因名：
    BCAT1
  • 货号：
    50181
  • 应用：
    WB, IC
  • 物种反应性：
    H, Mk, R, B, D, P, Rb, S
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunocytochemical analysis of ECA39 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of ECA39 expression in Jurkat (A), Ramos (B), NIH3T3 (C), PC12 (D) whole cell lysates.
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