Flow cytometric analysis of Casein kinase 1 epsilon expression in HT-1080 cells using anti-Casein kinase 1 epsilon antibody (Cat# 61250, 1:2,000). Green, isotype control; red, Casein kinase 1 epsilon.
Western blotting analysis using anti-casein kinase 1 epsilon antibody (Cat#61250). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-casein kinase 1 epsilon antibody (Cat#61250). Casein kinase 1 epsilon expression in wild type (WT) and casein kinase 1 epsilon (CSNK1E) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Casein kinase 1 epsilon knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Casein kinase 1 epsilon antibody (Cat#61250, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Casein kinase 1 epsilon antibody (Cat#61250, 1:1,000), Top panel: wild-type (WT); Bottom panal: Casein kinase 1 epsilon shRNA knockdown (KD). Nuclei were stained blue with DAPI; Casein kinase 1 epsilon was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
