Western blotting analysis using anti-pseudouridine synthase 7 antibody (Cat#5030). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pseudouridine synthase 7 antibody (Cat#5030, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-Pseudouridine synthase 7 antibody (Cat#5030, 1:1,000) . Nuclei were stained blue with DAPI; Pseudouridine synthase 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-pseudouridine synthase 7 antibody (Cat#5030). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pseudouridine synthase 7 antibody (Cat#5030, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Pseudouridine synthase 7 expression in HepG2 cells using anti-Pseudouridine synthase 7 antibody (Cat#5030, 1:1,000). Green, isotype control; red, Pseudouridine synthase 7.
