Flow cytometric analysis of HGS expression in HT-1080 cells using anti-HGS antibody (Cat#5047, 1:2,000). Green, isotype control; red, HGS.
Immunocytochemical staining of HT-1080 cells with anti-HGS antibody (Cat#5047, 1:1,000). Nuclei were stained blue with DAPI; HGS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-HGS antibody (Cat#5047). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HGS antibody (Cat#5047, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HGS expression in HT-1080 cells using anti-HGS antibody (Cat#5047, 1:2,000). Green, isotype control; red, HGS.
Immunocytochemical staining of HT-1080 cells with anti-HGS antibody (Cat#5047, 1:1,000). Nuclei were stained blue with DAPI; HGS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-HGS antibody (Cat#5047). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HGS antibody (Cat#5047, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HGS expression in HT-1080 cells using anti-HGS antibody (Cat#5047, 1:2,000). Green, isotype control; red, HGS.
Immunocytochemical staining of HT-1080 cells with anti-HGS antibody (Cat#5047, 1:1,000). Nuclei were stained blue with DAPI; HGS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-HGS antibody (Cat#5047). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HGS antibody (Cat#5047, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
