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Anti-PCNA Rabbit Polyclonal Antibody #50546

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Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of PCNA expression in H9C2 (A), 3T3L1 (B), HEK293T (C), A549 (D), HCT116 (E) whole cell lysates.
  • 基因名：
    PCNA
  • 货号：
    50546
  • 应用：
    WB, IHC
  • 物种反应性：
    H, M, R, B, C, D, Mk, P, Rb
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-PCNA Rabbit Polyclonal Antibody
Aliases Proliferating cell nuclear antigen; PCNA; Cyclin
Background

Gene Name: PCNA

NCBI Gene Entry: 5111

UniProt Entry: P12004
Application Information

Molecular Weight: Predicted, 28 kDa; observed, 36 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, dog, monkey, pig, rabbit

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human PCNA
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of PCNA expression in H9C2 (A), 3T3L1 (B), HEK293T (C), A549 (D), HCT116 (E) whole cell lysates.
Citation(0)
  • 基因名：
    PCNA
  • 货号：
    50546
  • 应用：
    WB, IHC
  • 物种反应性：
    H, M, R, B, C, D, Mk, P, Rb
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of PCNA expression in H9C2 (A), 3T3L1 (B), HEK293T (C), A549 (D), HCT116 (E) whole cell lysates.
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