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Anti-SMYD3 Rabbit Monoclonal Antibody #4393

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Flow cytometric analysis of SMYD3 expression in HepG2 cells using anti-SMYD3 antibody (Cat#4393, 1:2,000). Green, isotype control; red, SMYD3.

Immunocytochemical staining of HepG2 cells with anti-SMYD3 antibody (Cat#4393, 1:1,000). Nuclei were stained blue with DAPI; SMYD3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium . Scale bar, 20 μm.

Western blotting analysis using anti-SMYD3 antibody (Cat#4393). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMYD3 antibody (Cat#4393, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

SMYD3
货号：

4393
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-SMYD3 Rabbit Monoclonal Antibody
Aliases	SMYD3; SET And MYND Domain Containing 3; ZNFN3A1; ZMYND1; KMT3E; Zinc Finger MYND Domain-Containing Protein 1; Histone-Lysine N-Methyltransferase SMYD3 ; SET And MYND Domain-Containing Protein 3; Zinc Finger, MYND Domain Containing 1; Zinc Finger Protein, Subfamily 3A (MYND Domain Containing), 1; EC 2.1.1.354; EC 2.1.1.43; BA74P14.1; EC 2.1.1
Background	Gene Name: SMYD3 NCBI Gene Entry: 64754 UniProt Entry: Q9H7B4
Application Information	Molecular Weight: Predicted, 49 kDa; observed, 42 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10995 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SMYD3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of SMYD3 expression in HepG2 cells using anti-SMYD3 antibody (Cat#4393, 1:2,000). Green, isotype control; red, SMYD3.
Immunocytochemical staining of HepG2 cells with anti-SMYD3 antibody (Cat#4393, 1:1,000). Nuclei were stained blue with DAPI; SMYD3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium . Scale bar, 20 μm.
Western blotting analysis using anti-SMYD3 antibody (Cat#4393). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMYD3 antibody (Cat#4393, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

SMYD3
货号：

4393
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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