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Anti-SOD1 Rabbit Polyclonal Antibody #50653

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Immunocytochemical analysis of SOD1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Western blotting analysis of SOD1 expression in mouse liver (A), mouse testis (B), rat liver (C), rat testis (D) whole cell lysates.

基因名：

SOD1
货号：

50653
应用：

WB, IC
物种反应性：

H, M, R, B, Mk, Rb, S

规格：

20 μL，50 μL，100 μL

价格：

¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information

Product Name	Anti-SOD1 Rabbit Polyclonal Antibody
Aliases	Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1
Background	Gene Name: SOD1 NCBI Gene Entry: 6647 UniProt Entry: P00441
Application Information	Molecular Weight: Predicted, 15 kDa; observed, 16 kDa Clonality: Rabbit polyclonal antibody Species Reactivity: Human, mouse, rat, bovine, monkey, rabbit, sheep Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SOD1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:1,000 Immunocytochemistry (IC): 1:50-1:200
Note	This product is for research use only.

Picture

Immunocytochemical analysis of SOD1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of SOD1 expression in mouse liver (A), mouse testis (B), rat liver (C), rat testis (D) whole cell lysates.

Citation(0)

基因名：

SOD1
货号：

50653
应用：

WB, IC
物种反应性：

H, M, R, B, Mk, Rb, S

规格：

20 μL，50 μL，100 μL

价格：

¥ 560.00，¥ 1,100.00，¥ 2,000.00

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