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Anti-Keratin 7 Rabbit Monoclonal Antibody #5325

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Western blotting analysis using anti-keratin 7 antibody (Cat#5325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-keratin 7 antibody (Cat#5325, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-glial fibrillary acidic protein antibody (Cat#5325, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名：
    KRT7
  • 货号：
    5325
  • 应用：
    WB
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Keratin 7 Rabbit Monoclonal Antibody
Aliases KRT7; Keratin 7; CK-7; SCL; K7; Keratin, Type II Cytoskeletal 7; Sarcolectin; K2C7; CK7; Keratin, 55K Type II Cytoskeletal; Type-II Keratin Kb7; Keratin 7, Type II; Cytokeratin 7; Keratin, Simple Epithelial Type I, K7; Type II Mesothelial Keratin K7; Cytokeratin-7; Keratin-7
Background

Gene Name: KRT7

NCBI Gene Entry: 3855

UniProt Entry: P08729
Application Information

Molecular Weight: Predicted, 51 kDa; observed, 54 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB965

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human Cytokeratin 7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-keratin 7 antibody (Cat#5325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-keratin 7 antibody (Cat#5325, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-glial fibrillary acidic protein antibody (Cat#5325, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
  • 基因名：
    KRT7
  • 货号：
    5325
  • 应用：
    WB
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Western blotting analysis using anti-keratin 7 antibody (Cat#5325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-keratin 7 antibody (Cat#5325, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-glial fibrillary acidic protein antibody (Cat#5325, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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