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Anti-B4GALT5 Rabbit Polyclonal Antibody #50793

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Immunocytochemical analysis of B4GALT5 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of B4GALT5 expression in K562 (A), A549 (B), mouse heart (C), rat brain (D) whole cell lysates.
  • 基因名：
    B4GALT5
  • 货号：
    50793
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, Mk
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-B4GALT5 Rabbit Polyclonal Antibody
Aliases Beta-1,4-galactosyltransferase 5; Beta-1,4-GalTase 5; Beta4Gal-T5; b4Gal-T5; Beta-1,4-GalT II; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5
Background

Gene Name: B4GALT5

NCBI Gene Entry: 9334

UniProt Entry: O43286
Application Information

Molecular Weight: Predicted, 45 kDa; observed, 45 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, monkey

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human B4GALT5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:50-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of B4GALT5 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of B4GALT5 expression in K562 (A), A549 (B), mouse heart (C), rat brain (D) whole cell lysates.
Citation(0)
  • 基因名：
    B4GALT5
  • 货号：
    50793
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, Mk
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunocytochemical analysis of B4GALT5 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of B4GALT5 expression in K562 (A), A549 (B), mouse heart (C), rat brain (D) whole cell lysates.
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