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Anti-UBR7 Mouse Monoclonal Antibody #5085

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Flow cytometric analysis of UBR7 expression in HepG2 cells using anti-UBR7 antibody (Cat#5085, 1:2,000) . Green, isotype control; red, UBR7.

Immunocytochemical staining of HepG2 cells with anti-UBR7 antibody (Cat#5085, 1:1,000) . Nuclei were stained blue with DAPI; UBR7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-UBR7 antibody (Cat#5085). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBR7 antibody (Cat#5085, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

UBR7
货号：

5085
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-UBR7 Mouse Monoclonal Antibody
Aliases	UBR7; Ubiquitin Protein Ligase E3 Component N-Recognin 7; C14orf130; Ubiquitin Protein Ligase E3 Component N-Recognin 7 (Putative); Putative E3 Ubiquitin-Protein Ligase UBR7; RING-Type E3 Ubiquitin Transferase UBR7; N-Recognin-7; Chromosome 14 Open Reading Frame 130; EC 2.3.2.27; LICAS
Background	Gene Name: UBR7 NCBI Gene Entry: 55148 UniProt Entry: Q8N806
Application Information	Molecular Weight: Predicted, 48 kDa; observed, 51 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB14240 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human UBR7
Isotype	Mouse IgG2a
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of UBR7 expression in HepG2 cells using anti-UBR7 antibody (Cat#5085, 1:2,000) . Green, isotype control; red, UBR7.
Immunocytochemical staining of HepG2 cells with anti-UBR7 antibody (Cat#5085, 1:1,000) . Nuclei were stained blue with DAPI; UBR7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-UBR7 antibody (Cat#5085). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBR7 antibody (Cat#5085, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

UBR7
货号：

5085
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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