Flow cytometric analysis of PAM expression in HT-1080 cells using anti-PAM antibody (Cat#5125, 1:2,000) . Green, isotype control; red, PAM.
Western blotting analysis using anti-PAM antibody (Cat#5125). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAM antibody (Cat#5125, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human PAM
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:2,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of PAM expression in HT-1080 cells using anti-PAM antibody (Cat#5125, 1:2,000) . Green, isotype control; red, PAM.
Western blotting analysis using anti-PAM antibody (Cat#5125). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAM antibody (Cat#5125, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PAM expression in HT-1080 cells using anti-PAM antibody (Cat#5125, 1:2,000) . Green, isotype control; red, PAM.
Western blotting analysis using anti-PAM antibody (Cat#5125). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAM antibody (Cat#5125, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
