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Anti-ZNF598 Mouse Monoclonal Antibody #5129

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Flow cytometric analysis of ZNF598 expression in HT-1080 cells using anti-ZNF598 antibody (Cat#5129, 1:2,000) . Green, isotype control; red, ZNF598.
Immunocytochemical staining of HT-1080 cells with anti-ZNF598 antibody (Cat#5129, 1:1,000) . Nuclei were stained blue with DAPI; ZNF598 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-ZNF598 antibody (Cat#5129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZNF598 antibody (Cat#5129, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    ZNF598
  • 货号:
    5129
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-ZNF598 Mouse Monoclonal Antibody
Aliases ZNF598; Zinc Finger Protein 598, E3 Ubiquitin Ligase; HEL2; E3 Ubiquitin-Protein Ligase ZNF598; FLJ00086; Zinc Finger Protein 598; EC 2.3.2.27
Background

Gene Name: ZNF598

NCBI Gene Entry: 90850

UniProt Entry: Q86UK7

Application Information

Molecular Weight: Predicted, 99 kDa; observed, 99 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB15860

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human ZNF598
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ZNF598 expression in HT-1080 cells using anti-ZNF598 antibody (Cat#5129, 1:2,000) . Green, isotype control; red, ZNF598.
  • Immunocytochemical staining of HT-1080 cells with anti-ZNF598 antibody (Cat#5129, 1:1,000) . Nuclei were stained blue with DAPI; ZNF598 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-ZNF598 antibody (Cat#5129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZNF598 antibody (Cat#5129, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of ZNF598 expression in HT-1080 cells using anti-ZNF598 antibody (Cat#5129, 1:2,000) . Green, isotype control; red, ZNF598.
Immunocytochemical staining of HT-1080 cells with anti-ZNF598 antibody (Cat#5129, 1:1,000) . Nuclei were stained blue with DAPI; ZNF598 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-ZNF598 antibody (Cat#5129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZNF598 antibody (Cat#5129, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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