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Anti-p21 Rabbit Polyclonal Antibody #51727

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Western blotting analysis using anti-p21 antibody (Cat#51727). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p21 antibody (Cat#51727, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with anti-CDKN1A antibody (Cat#51727, 1:1,000). Nuclei were stained blue with DAPI; CDKN1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • 基因名:
    CDKN1A
  • 货号:
    51727
  • 应用:
    WB, IHC, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-p21 Rabbit Polyclonal Antibody
Aliases CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
Background

Gene Name: CDKN1A

NCBI Gene Entry: 1026

UniProt Entry: P38936

Application Information

Molecular Weight: Predicted, 18 kDa; observed, 18 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human p21
Isotype Rabbit IgG
Storage Buffer Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:50-1:200
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-p21 antibody (Cat#51727). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p21 antibody (Cat#51727, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with anti-CDKN1A antibody (Cat#51727, 1:1,000). Nuclei were stained blue with DAPI; CDKN1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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Western blotting analysis using anti-p21 antibody (Cat#51727). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p21 antibody (Cat#51727, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with anti-CDKN1A antibody (Cat#51727, 1:1,000). Nuclei were stained blue with DAPI; CDKN1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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