Western blotting analysis using anti-IL-3R beta antibody (Cat#52324). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IL-3R beta antibody (Cat#52324, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IL-3R beta antibody (Cat#52324). The picture on the right is blocked with the synthesized peptide.
Immunohistochemical staining of paraffin-embedded Human brain with IL-3R beta antibody (Cat#52324, 1:100), 4° overnight. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen
A synthesized peptide derived from human IL-3R beta
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry (IHC): 1:200-1:1,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-IL-3R beta antibody (Cat#52324). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IL-3R beta antibody (Cat#52324, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IL-3R beta antibody (Cat#52324). The picture on the right is blocked with the synthesized peptide.
Immunohistochemical staining of paraffin-embedded Human brain with IL-3R beta antibody (Cat#52324, 1:100), 4° overnight. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blotting analysis using anti-IL-3R beta antibody (Cat#52324). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IL-3R beta antibody (Cat#52324, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IL-3R beta antibody (Cat#52324). The picture on the right is blocked with the synthesized peptide.
Immunohistochemical staining of paraffin-embedded Human brain with IL-3R beta antibody (Cat#52324, 1:100), 4° overnight. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
