Western blotting analysis using anti-LC3B antibody (Cat#52346). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-LC3B antibody (Cat#52346, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical analysis of Mouse-lung tissue. 1,LC3B Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunocytochemical analysis of Hela cell. 1, LC3B Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1,000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
Human brain was stained with anti-LC3B antibody.
Mouse brain was stained with anti-LC3B antibody.
Rat brain was stained with anti-LC3B antibody.