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Anti-LC3B Rabbit Monoclonal Antibody #52346

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Western blotting analysis using anti-LC3B antibody (Cat#52346). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-LC3B antibody (Cat#52346, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical analysis of Mouse-lung tissue. 1,LC3B Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunocytochemical analysis of Hela cell. 1, LC3B Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1,000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
Human brain was stained with anti-LC3B antibody.
Mouse brain was stained with anti-LC3B antibody.
Rat brain was stained with anti-LC3B antibody.
  • 基因名:
    MAP1LC3B
  • 货号:
    52346
  • 应用:
    WB, IC, IHC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-LC3B Rabbit Monoclonal Antibody
Aliases MAP1LC3B; Microtubule Associated Protein 1 Light Chain 3 Beta; ATG8F; Microtubule-Associated Proteins 1A/1B Light Chain 3B; Autophagy-Related Ubiquitin-Like Modifier LC3 B; MAP1 Light Chain 3-Like Protein; MAP1A/MAP1B Light Chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-Associated Protein 1 Light Chain 3 Beta; Autophagy-Related Protein LC3 B; MAP1A/1BLC3; MAP1LC3B-A; MAP1ALC3; LC3B
Background

Gene Name: MAP1LC3B

NCBI Gene Entry: 81631

UniProt Entry: Q9GZQ8

Application Information

Molecular Weight: Predicted, 14,16 kDa; observed, 14,16 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB15760

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), immunohistochemistry (IHC)

Isotype Rabbit IgG,Kappa
Storage Buffer Supplied in PBS containing 50% glycerol, 0.05% BSA and 0.05% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:200-1:1,000
Immunohistochemistry (IHC): 1:200-1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-LC3B antibody (Cat#52346). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-LC3B antibody (Cat#52346, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical analysis of Mouse-lung tissue. 1,LC3B Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunocytochemical analysis of Hela cell. 1, LC3B Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1,000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
  • Human brain was stained with anti-LC3B antibody.
  • Mouse brain was stained with anti-LC3B antibody.
  • Rat brain was stained with anti-LC3B antibody.
Citation(0)
Western blotting analysis using anti-LC3B antibody (Cat#52346). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-LC3B antibody (Cat#52346, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical analysis of Mouse-lung tissue. 1,LC3B Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunocytochemical analysis of Hela cell. 1, LC3B Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1,000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
Human brain was stained with anti-LC3B antibody.
Mouse brain was stained with anti-LC3B antibody.
Rat brain was stained with anti-LC3B antibody.
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