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KD-Validated Anti-CNP Mouse Monoclonal Antibody #63774

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Western blotting analysis using anti-CNP antibody (Cat#63774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CNP antibody (Cat#63774). CNP expression in wild-type (WT) and CNP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CNP
  • 货号:
    63774
  • 应用:
    WB, IC, FCM, IHC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-CNP Mouse Monoclonal Antibody
Aliases CNP; 2',3'-Cyclic Nucleotide 3' Phosphodiesterase; 2',3'-Cyclic-Nucleotide 3'-Phosphodiesterase; EC 3.1.4.37; CNPase; 2', 3' Cyclic Nucleotide 3' Phosphohydrolase; HLD20; CNP1
Background

Gene Name: CNP

NCBI Gene Entry: 1267

UniProt Entry: P09543

Application Information

Molecular Weight: Predicted, 48 kDa; Observed, 40 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8575

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CNPase
Isotype Mouse IgG1/Kappa
Storage Buffer Supplied in TBS (pH 7.4) containing 50% glycerol, 1% BSA and 0.02% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-CNP antibody (Cat#63774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-CNP antibody (Cat#63774). CNP expression in wild-type (WT) and CNP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-CNP antibody (Cat#63774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CNP antibody (Cat#63774). CNP expression in wild-type (WT) and CNP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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