Western blotting analysis using anti-BGLAP antibody (Cat#52451). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-BGLAP antibody (Cat#52451, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical analysis of A549. Primary antibody was diluted at 1:200(4°C overnight). Goat Anti Rabbit IgG (H&L)-AF 488 secondary antibody was diluted at 1:1,000 (room temperature, 50min). Picture B: DAPI (blue) 10min.
BGLAP; Bone Gamma-Carboxyglutamate Protein; Osteocalcin; OCN; Gamma-Carboxyglutamic Acid-Containing Protein; Bone Gla Protein; BGP; Bone Gamma-Carboxyglutamate (Gla) Protein (Osteocalcin); Bone Gamma-Carboxyglutamate (Gla) Protein; OC
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human OSTCN
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunocytochemistry (IC): 1:100-1:300
Note
This product is for research use only.
Picture
Western blotting analysis using anti-BGLAP antibody (Cat#52451). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-BGLAP antibody (Cat#52451, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical analysis of A549. Primary antibody was diluted at 1:200(4°C overnight). Goat Anti Rabbit IgG (H&L)-AF 488 secondary antibody was diluted at 1:1,000 (room temperature, 50min). Picture B: DAPI (blue) 10min.
Western blotting analysis using anti-BGLAP antibody (Cat#52451). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-BGLAP antibody (Cat#52451, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical analysis of A549. Primary antibody was diluted at 1:200(4°C overnight). Goat Anti Rabbit IgG (H&L)-AF 488 secondary antibody was diluted at 1:1,000 (room temperature, 50min). Picture B: DAPI (blue) 10min.
