Immunocytochemical analysis of HEK293T cells transfected with Luciferase. Luciferase antibody (green) was diluted at 1:200(4°C,overnight). Dylight 488 labled secondary antibody was diluted at 1:300 room temperature, 30min. DAPI (blue) 10min.
Western blotting analysis of over-expressed Luciferase protein using antibody diluted at 1:1,000.
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
Recombinant protein of Luciferase
Storage Buffer
Supplied in PBS (pH7.4) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:2,000 Immunocytochemistry (IC): 1:50-1:200
Note
This product is for research use only.
Picture
Immunocytochemical analysis of HEK293T cells transfected with Luciferase. Luciferase antibody (green) was diluted at 1:200(4°C,overnight). Dylight 488 labled secondary antibody was diluted at 1:300 room temperature, 30min. DAPI (blue) 10min.
Western blotting analysis of over-expressed Luciferase protein using antibody diluted at 1:1,000.
Immunocytochemical analysis of HEK293T cells transfected with Luciferase. Luciferase antibody (green) was diluted at 1:200(4°C,overnight). Dylight 488 labled secondary antibody was diluted at 1:300 room temperature, 30min. DAPI (blue) 10min.
Western blotting analysis of over-expressed Luciferase protein using antibody diluted at 1:1,000.
