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KD-Validated Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody #62329

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Flow cytometric analysis of Phospho-mTOR (S2481) expression in HepG2 cells using Phospho-mTOR (S2481) antibody (Cat#62329, 1:2,000). Green, isotype control; red, Phospho-mTOR (S2481).
Immunocytochemical staining of HepG2 cells with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (S2481) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-mTOR (S2481) antibody (Cat#62329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-mTOR (S2481) antibody (Cat#62329). Phospho-mTOR (S2481) expression in wild type (WT) and Phospho-mTOR (S2481) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    MTOR
  • 货号：
    62329
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
Aliases Mechanistic Target Of Rapamycin Kinase; RAFT1; RAPT1; Rapamycin And FKBP12 Target 1; Mammalian Target Of Rapamycin; FRAP1; FRAP2; FRAP; FK506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; Mechanistic Target Of Rapamycin (Serine/Threonine Kinase); FK506 Binding Protein 12-Rapamycin Associated Protein 2; FKBP12-Rapamycin Complex-Associated Protein 1; Serine/Threonine-Protein Kinase MTOR; Rapamycin Associated Protein FRAP2; FKBP-Rapamycin Associated Protein; Mechanistic Target Of Rapamycin; Rapamycin Target Protein 1; FLJ44809; DJ576K7.1 (FK506 Binding Protein 12-Rapamycin Associated Protein 1); FK506 Binding Protein 12-Rapamycin Associated Protein 1; FKBP12-Rapamycin Complex-Associated Protein; Rapamycin Target Protein; EC 2.7.11.1; MTOR; SKS
Background

Gene Name: MTOR

NCBI Gene Entry: 2475

UniProt Entry: P42345
Application Information

Molecular Weight: Predicted, 289 kDa, observed, 255 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB325

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-mTOR (S2481)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-mTOR (S2481) expression in HepG2 cells using Phospho-mTOR (S2481) antibody (Cat#62329, 1:2,000). Green, isotype control; red, Phospho-mTOR (S2481).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (S2481) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Phospho-mTOR (S2481) antibody (Cat#62329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Phospho-mTOR (S2481) antibody (Cat#62329). Phospho-mTOR (S2481) expression in wild type (WT) and Phospho-mTOR (S2481) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    MTOR
  • 货号：
    62329
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-mTOR (S2481) expression in HepG2 cells using Phospho-mTOR (S2481) antibody (Cat#62329, 1:2,000). Green, isotype control; red, Phospho-mTOR (S2481).
Immunocytochemical staining of HepG2 cells with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (S2481) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-mTOR (S2481) antibody (Cat#62329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-mTOR (S2481) antibody (Cat#62329). Phospho-mTOR (S2481) expression in wild type (WT) and Phospho-mTOR (S2481) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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