Immunofluorescent analysis of Cytokeratin 14 staining in
MCF7 cells. Formalin-fixed cells were permeabilized with 0.1%
Triton X-100 in TBS for 5-10 minutes and blocked with 3%
BSA-PBS for 30 minutes at room temperature. Cells were
probed with the primary antibody in 3% BSA-PBS and
incubated overnight at 4 °C in a humidified chamber. Cells
were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room
temperature in the dark.

Immunohistochemical analysis of Cytokeratin 14 staining in
human breast cancer formalin fixed paraffin embedded tissue
section. The section was pre-treated using heat mediated
antigen retrieval with sodium citrate buffer (pH 6.0). The
section was then incubated with the antibody at room
temperature and detected using an HRP conjugated compact
polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted
with DPX.

Western blotting analysis of Cytokeratin 14 expression in MCF7
(A), mouse brain (B), rat brain (C) whole cell lysates.