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KD-Validated Anti-RBBP7 Mouse Monoclonal Antibody #65038

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Western blotting analysis using anti-RBBP7 antibody (Cat#65038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RBBP7 antibody (Cat#65038). RBBP7 expression in wild-type (WT) and RBBP7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-RBBP7 antibody (Cat#65038, 1:1,000) . Nuclei were stained blue with DAPI; RBBP7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-RBBP7 antibody (Cat#65038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RBBP7 antibody (Cat#65038). RBBP7 expression in wild-type (WT) and RBBP7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of RBBP7 expression in HepG2 cells using anti-RBBP7 antibody (Cat#65038, 1:1,000). Green, isotype control; red, RBBP7.
  • 基因名：
    RBBP7
  • 货号：
    65038
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-RBBP7 Mouse Monoclonal Antibody
Aliases RBBP7; RB Binding Protein 7, Chromatin Remodeling Factor; Histone Acetyltransferase Type B Subunit 2; Retinoblastoma-Binding Protein P46; Retinoblastoma-Binding Protein 7; RbAp46; G1/S Transition Control Protein-Binding Protein RbAp46; Nucleosome-Remodeling Factor Subunit RBAP46; Retinoblastoma-Binding Protein RbAp46; Histone-Binding Protein RBBP7; RBBP-7; Retinoblastoma Binding Protein 7; RB Binding Protein 7; RBAP46
Background

Gene Name: RBBP7

NCBI Gene Entry: 5931

UniProt Entry: Q16576
Application Information

Molecular Weight: Predicted, 48 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13975

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen Recombinant protein of human RBBP7
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-RBBP7 antibody (Cat#65038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-RBBP7 antibody (Cat#65038). RBBP7 expression in wild-type (WT) and RBBP7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with anti-RBBP7 antibody (Cat#65038, 1:1,000) . Nuclei were stained blue with DAPI; RBBP7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-RBBP7 antibody (Cat#65038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-RBBP7 antibody (Cat#65038). RBBP7 expression in wild-type (WT) and RBBP7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of RBBP7 expression in HepG2 cells using anti-RBBP7 antibody (Cat#65038, 1:1,000). Green, isotype control; red, RBBP7.
Citation(0)
  • 基因名：
    RBBP7
  • 货号：
    65038
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-RBBP7 antibody (Cat#65038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RBBP7 antibody (Cat#65038). RBBP7 expression in wild-type (WT) and RBBP7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-RBBP7 antibody (Cat#65038, 1:1,000) . Nuclei were stained blue with DAPI; RBBP7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-RBBP7 antibody (Cat#65038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RBBP7 antibody (Cat#65038). RBBP7 expression in wild-type (WT) and RBBP7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP7 antibody (Cat#65038, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of RBBP7 expression in HepG2 cells using anti-RBBP7 antibody (Cat#65038, 1:1,000). Green, isotype control; red, RBBP7.
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