Immunohistochemical analysis of paraffin-embedded human tonsil. Antibody was diluted at 1:200 (4℃ overnight). Tris-EDTA, pH9.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 45min).
Western blotting analysis using anti-SH2D5 antibody (Cat#52620). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SH2D5 antibody (Cat#52620, 1:2,000).
Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen
A synthesized peptide derived from human SH2D5
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry(IHC): 1:50-1:300
Note
This product is for research use only.
Picture
Immunohistochemical analysis of paraffin-embedded human tonsil. Antibody was diluted at 1:200 (4℃ overnight). Tris-EDTA, pH9.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 45min).
Western blotting analysis using anti-SH2D5 antibody (Cat#52620). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SH2D5 antibody (Cat#52620, 1:2,000).
Immunohistochemical analysis of paraffin-embedded human tonsil. Antibody was diluted at 1:200 (4℃ overnight). Tris-EDTA, pH9.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 45min).
Western blotting analysis using anti-SH2D5 antibody (Cat#52620). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SH2D5 antibody (Cat#52620, 1:2,000).
