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KD-Validated Anti-PTPN11 Rabbit Monoclonal Antibody #61507

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Flow cytometric analysis of PTPN11 expression in HepG2 cells using PTPN11 antibody (Cat#61507, 1:2,000). Green, isotype control; red, PTPN11.
Immunocytochemical staining of HepG2 cells with PTPN11 antibody (Cat#61507, 1:1,000). Nuclei were stained blue with DAPI; PTPN11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PTPN11 antibody (Cat#61507). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTPN11 antibody (Cat#61507, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PTPN11, protein tyrosine phosphatase non-receptor type 11.
Western blotting analysis using anti-PTPN11 antibody (Cat#61507). PTPN11 expression in wild type (WT) and PTPN11 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PTPN11 antibody (Cat#61507, 1:5000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of PTPN11 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PTPN11 antibody (Cat#61507, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名:
    PTPN11
  • 货号:
    61507
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-PTPN11 Rabbit Monoclonal Antibody
Aliases PTPN11; Protein Tyrosine Phosphatase Non-Receptor Type 11; SH-PTP2; PTP2C; BPTP3; SHP-2; SHP2; SH2 Domain-Containing Protein Tyrosine Phosphatase 2; Tyrosine-Protein Phosphatase Non-Receptor Type 11; Protein-Tyrosine Phosphatase 1D; Protein-Tyrosine Phosphatase 2C; EC 3.1.3.48; SH-PTP3; PTP-1D; NS1; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Noonan Syndrome 1; METCDS; SHPTP2; PTP-2C; JMML; Shp2; CFC
Background

Gene Name: PTPN11

NCBI Gene Entry: 5781

UniProt Entry: Q06124

Application Information

Molecular Weight: Predicted, 68 kDa, observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2665

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human SHP2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PTPN11 expression in HepG2 cells using PTPN11 antibody (Cat#61507, 1:2,000). Green, isotype control; red, PTPN11.
  • Immunocytochemical staining of HepG2 cells with PTPN11 antibody (Cat#61507, 1:1,000). Nuclei were stained blue with DAPI; PTPN11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-PTPN11 antibody (Cat#61507). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTPN11 antibody (Cat#61507, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PTPN11, protein tyrosine phosphatase non-receptor type 11.
  • Western blotting analysis using anti-PTPN11 antibody (Cat#61507). PTPN11 expression in wild type (WT) and PTPN11 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PTPN11 antibody (Cat#61507, 1:5000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of PTPN11 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PTPN11 antibody (Cat#61507, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
Flow cytometric analysis of PTPN11 expression in HepG2 cells using PTPN11 antibody (Cat#61507, 1:2,000). Green, isotype control; red, PTPN11.
Immunocytochemical staining of HepG2 cells with PTPN11 antibody (Cat#61507, 1:1,000). Nuclei were stained blue with DAPI; PTPN11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PTPN11 antibody (Cat#61507). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTPN11 antibody (Cat#61507, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PTPN11, protein tyrosine phosphatase non-receptor type 11.
Western blotting analysis using anti-PTPN11 antibody (Cat#61507). PTPN11 expression in wild type (WT) and PTPN11 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PTPN11 antibody (Cat#61507, 1:5000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of PTPN11 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PTPN11 antibody (Cat#61507, 1:2,000) and analyzed using BD flow cytometer.
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