Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TFCP2L1 antibody (Cat#52629, 1:200) overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
Western blotting analysis using anti-TFCP2L1 antibody (Cat#52629). Total lysates (40 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-TFCP2L1 antibody (Cat#52629, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human CRTR1
Isotype
Rabbit IgG
Storage Buffer
Supplied in TBS (pH 7.4) containing 50% glycerol, 1% BSA and 0.02% Proclin 300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry(IHC): 1:100-1:500 Immunocytochemistry (IC): 1:100-1:500
Note
This product is for research use only.
Picture
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TFCP2L1 antibody (Cat#52629, 1:200) overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
Western blotting analysis using anti-TFCP2L1 antibody (Cat#52629). Total lysates (40 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-TFCP2L1 antibody (Cat#52629, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TFCP2L1 antibody (Cat#52629, 1:200) overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
Western blotting analysis using anti-TFCP2L1 antibody (Cat#52629). Total lysates (40 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-TFCP2L1 antibody (Cat#52629, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
