Immunocytochemical analysis of HeLa. Antibody incubation with DNA-RNA Hybrid antibody (Cat#52748, 1:100), 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid antibody (Cat#52748, 1:200), overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
