Western blotting analysis using anti-Phospho-Smad1/5/9 (Ser463+Ser465) antibody (Cat#U2211). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Smad1/5/9 (Ser463+Ser465) antibody (Cat#U2211, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
A synthesized peptide derived from human Phospho-Smad1/5/9 (Ser463+Ser465)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-Phospho-Smad1/5/9 (Ser463+Ser465) antibody (Cat#U2211). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Smad1/5/9 (Ser463+Ser465) antibody (Cat#U2211, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Smad1/5/9 (Ser463+Ser465) antibody (Cat#U2211). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Smad1/5/9 (Ser463+Ser465) antibody (Cat#U2211, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
